FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13514519
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGAGGTTGCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTG534380.39541177899117236No Hit
GGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCTA297670.22025941137823699No Hit
GTGAATGTTATAATTAAGGAGATTTGTAGGGAGATTAGTATAGAGAGGTA255240.18886354741889075No Hit
GTCGGAGGAAAAGGTTGGGGAACAGCTAAATAGGTTGTTGTTGATTTGGT248510.18388371794808234No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT244990.1812791117464114No Hit
GTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATTATTCCTT235850.17451601496139077No Hit
GGGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCT211840.15674993686419766No Hit
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGT208980.15463369432534002No Hit
GTTTGGTATTGGGTTATGGCAGGGGGTTTTATATTGATAATTGTTGTGAT204340.15120034978677377No Hit
GAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTTGG199370.14752282341680084No Hit
GGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAA194290.14376390310302573No Hit
GTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAATAAAA188970.13982739600277302No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG187120.13845849785700845No Hit
GTGGTGTTGAGGTTGCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAG185130.13698600741913197No Hit
GGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGTT180180.1333232799480322No Hit
GGCGTTTGGTATTGGGTTATGGCAGGGGGTTTTATATTGATAATTGTTGT175670.1299861282521413No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC151550.11213865621114597No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT144040.10658166968428547No Hit
TGTGAATGTTATAATTAAGGAGATTTGTAGGGAGATTAGTATAGAGAGGT140270.10379207724670039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTGCG137450.039.315866
GTCCGGT16200.035.8101771
GTTGCGG153600.035.212667
TGCGGTC153200.035.1527989
TTGCGGT168700.032.3673678
GTCCGTT13450.030.1573091
GACGAAG190600.027.37239594
GTCGGAG95500.025.6812611
ACCGCTG98400.025.17298392-93
TCAATCG22150.025.0336748
GTTGAGG325650.024.7228681
GTCGGAA31400.024.6337551
GTCGCGT6000.024.3683911
GTTATGA98350.023.9853278
GGGGGGT115150.023.9202271
GTCGGGT23900.023.8783251
AGGTTGC228050.023.6551935
TCGGAGG105200.022.8254782
GCGATTG19900.022.6690253
GGTCTTA102400.022.302374
Produced by FastQC (version 0.11.5)