FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13514519
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCT167330.12381498742204589No Hit
GGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTT140550.10399926183092421No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT138460.10245277689868207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCAT47700.029.105861
CGTCATC62700.022.5193962
CGACCGT6850.022.0602741
GTAGGTC25650.020.4356612
ACTACCG48450.019.4934847
TACTCTA78300.018.3704139
CGCCGTA8200.018.33475546-47
CTACCGT58400.017.0626668
GTTGACT56600.017.0202221
TCATCTA92050.016.178834
CCGTATG48950.016.123834
GTCTACC74600.015.8253261
GACGCTA59350.015.6028799
CAACCCG29550.015.4214173
TACCGTA63550.015.2374019
CGTATGG52500.014.9448985
TACTAGC98200.014.7375897
CATAAAT97450.014.3921641
CGCAAGG78850.014.36143194
AACCCGT30600.014.2781924
Produced by FastQC (version 0.11.5)