FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13544262
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCT168200.12418542996288762No Hit
GGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTT143310.10580864428050786No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT139110.10270770013161293No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC136200.10055918882845001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCAT44650.028.5922431
GTAGGTC27450.022.1393812
CGTCATC59200.021.4862292
CGCCGTA6800.020.73213446-47
CGACCGT6850.019.9437981
ACTACCG48600.019.7244037
TACTCTA71550.018.2583379
CTACCGT58050.017.0804088
TACCGTA62050.016.0553639
GACGCTA56500.016.0522149
CGGTAAT10550.015.6284531
CCGTATG47750.015.5480574
GTCTACC73850.015.5008741
CAACCCG27750.015.4088693
GTTGACT54950.015.0884761
CGCAAGG80450.015.01186894
TCATCTA91250.014.8818654
AGTAGGT40850.014.8764091
AACCCGT29500.014.4947834
GCCTATC70750.014.2124346
Produced by FastQC (version 0.11.5)