FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12126275
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG762090.6284617493830547No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT390430.321970267044084No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT175630.14483425454230586No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG159840.13181294338121147No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC124590.10274383518434144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTAG146050.050.3201035
TAGCATA147200.050.0521939
GCCAGTA154650.047.6737564
GTAGCAT157800.046.9885528
CAGTAGC162300.045.773716
AGTAGCA175150.042.3879667
TCACCCG100750.041.5098653
CACCCGG101800.041.2201964
ACCCGGC101450.040.8992235
TGCCAGT187850.039.5232163
CAGTTAT222200.032.43997694
CGACCTA17950.032.1993454
CGGCCCG132700.031.4791418
CCTGCCA261650.028.6768131
GCATATG163350.028.08887910-11
CGGACAC95050.027.9840812-13
CTGCCAG268050.027.7459982
CTTAGCG15050.027.4750358
CTCACCC157000.026.9408152
GCGATTT143800.026.89305594
Produced by FastQC (version 0.11.5)