FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12101236
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG376810.3113814159148702No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT256820.2122262552354156No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG225750.1865511919608873No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA184910.15280257322475158No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG183710.15181093898177012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTT63250.039.965311
CCCGTCG69600.032.6600381
TCGTATG18250.030.0025342-43
TATGCCG17050.029.36053546-47
TGTTACG16600.028.3071463
CGGCATG151500.025.6505723
GTTACGA19050.024.913264
GTATCGA22050.024.5071814
TTACGAC19800.023.9695765
TGTATTA173700.022.3993118
GCATGTA176050.022.1003155
CGACTTT130900.022.0769772
CATGTAT178750.021.792786
GTATTAG179050.021.730929
GCAGTTT177800.021.67736494
CGATCCG89950.021.15950882-83
CCGTCGG109650.021.084372
TCGACCG93200.020.62304930-31
GTTCGAC93300.020.52526328-29
GGCATGT194700.020.031634
Produced by FastQC (version 0.11.5)