FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12121571
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG381420.3146621836393979No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT257280.21224971581653898No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG230740.19035486406836208No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG185580.15309896712233093No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA180550.14894933998241647No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC121720.1004160269324826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTT62950.041.2674521
CCCGTCG70250.032.0799261
TCGTATG19200.030.96920442-43
TATGCCG18000.030.55320746-47
TGTTACG15400.025.3270323
CGGCATG147050.024.7025283
GTTACGA17500.022.8248464
TTACGAC17700.022.567032
CGACTTT134750.022.0402772
ACTAAAC62550.022.0164894
TGTATTA165700.021.6087178
GTTCGAC94550.021.52263528-29
CGATCCG93500.021.3866982-83
TCGACCG96100.021.37291730-31
GTATGCC28500.021.27604944-45
GCATGTA169350.021.2554635
CATGTAT170950.021.056526
CCGTCGG109500.020.899872
GCAGTTT173750.020.61283594
ACTTTTA150050.020.5757794
Produced by FastQC (version 0.11.5)