FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12557298
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1074050.8553193529372323No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1005350.8006101312559438No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC298910.2380368770415419No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT252000.2006801144641148No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG154960.12340234340221917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG201850.056.849733
ACCCGGC205400.055.867185
CACCCGG208800.055.092494
CCAGTAG190600.054.21435
GCCAGTA195750.052.835984
TAGCATA202650.050.9879579
CAGTAGC203400.050.8714876
GTAGCAT206400.050.1760068
AGTAGCA214600.048.2818037
CGGCCCG240950.047.9346058
TGCCAGT227750.045.639223
CTCACCC269100.043.1714742
GCGATTT262250.041.5161394
CTAGACG13100.038.464261
CCTGCCA274250.037.8107721
GGCCCGG306150.037.6795969
CCGGCCC314700.037.2544677
CAGTTAT269050.037.1833994
CCCGGCC313650.037.0199246
CGACCTA21100.036.3008354
Produced by FastQC (version 0.11.5)