FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12530712
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG580640.4633735098213095No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT245530.19594257692619543No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG221090.17643849766876774No Hit
CGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC193380.15432483006552222No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA185590.1481081043120295No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC179350.14312833939523947No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG164280.1311018879054917No Hit
CGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAG157000.1252921621692367No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA140130.11182924003041486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTT54100.043.324871
CGGCATG167400.033.2362443
GTTACGA13700.032.5850754
TTACGAC14350.030.7816335
CCCGTCG56700.030.1926821
TGTATTA191450.029.3800648
TGTTACG15250.028.9650143
GTATTAG194750.028.7866299
GCATGTA194150.028.7537675
CATGTAT198400.028.2087656
GCAGTTT207900.026.58730194
GGCATGT216300.025.9613384
ATGTATT221150.025.4556317
TGTCCGT34750.024.88768294
GTTCGAC96650.023.85047728-29
CGATCCG95850.023.55942282-83
TCGACCG99000.023.30852730-31
TCGGCAT254200.023.0518952
AAGTTCG101200.022.70767426-27
CGACTTT125000.021.9542472
Produced by FastQC (version 0.11.5)