FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12530712
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1082970.8642525660154029No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1016880.8115101520169007No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC295460.23578867665301062No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT256750.20489657730542368No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG156380.1247973778345556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG192250.057.258423
ACCCGGC198000.055.597615
CACCCGG203200.054.2424744
CCAGTAG182700.053.618735
GCCAGTA188750.051.948214
GTAGCAT197250.050.1363688
TAGCATA195500.050.1313559
CAGTAGC196650.049.9345746
AGTAGCA206750.047.7256977
CGGCCCG235350.047.1326568
TGCCAGT225500.043.606993
CTCACCC263650.042.174312
GCGATTT251950.040.74984794
CGACCTA21400.039.298364
CCCGGCC308150.036.3795476
GGCCCGG305650.036.340389
CCGGCCC311250.036.2611967
CAGTTAT266050.035.99397794
CCTGCCA276000.035.8374331
CGGACAC209450.033.14007612-13
Produced by FastQC (version 0.11.5)