FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12513122
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG579880.46341752282124316No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT247400.19771244937913973No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG224050.17905203833224034No Hit
CGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC197160.15756259708808082No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA186710.14921136387865475No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC180950.14460819610006198No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG163070.13031919612068035No Hit
CGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAG157110.12555619612755314No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA139760.11169075151668785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACTT54100.043.2195051
TTACGAC13300.035.684655
GTTACGA13650.035.1139144
CGGCATG168150.033.7305833
TGTTACG15400.030.8186863
CCCGTCG59200.029.1443731
GTATTAG195350.029.0559949
TGTATTA196150.029.0093528
GCATGTA197050.028.854975
CATGTAT203650.027.9659736
GCAGTTT217900.026.07811794
GGCATGT221500.025.839574
ATGTATT226250.025.108457
TACGACT20550.023.5525046
ACTAAAC76650.023.42385594
TCGGCAT256950.023.390372
GTTCGAC97050.023.00221428-29
TCGACCG98850.022.72739630-31
CGATCCG97900.022.36942782-83
AAGTTCG102650.021.81436526-27
Produced by FastQC (version 0.11.5)