FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12513122
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1093230.8736668594775948No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1016690.812499070975253No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC302520.24176220770483975No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT259880.2076859795660907No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG156500.125068707873223No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA125460.10026274817747322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG202800.057.462263
ACCCGGC208950.055.7921335
CACCCGG209850.055.509184
CCAGTAG186850.053.6145485
GCCAGTA190550.052.47594
TAGCATA199050.050.6896329
CAGTAGC199300.050.523656
GTAGCAT203350.049.776758
AGTAGCA212150.047.555057
CGGCCCG247500.047.3338788
TGCCAGT225650.044.4800153
CTCACCC271100.043.339992
GCGATTT263700.041.50446394
CGACCTA20150.038.0110444
GGCCCGG311550.037.770779
CCCGGCC317350.037.23746
CCGGCCC319850.037.2131547
CCTGCCA275650.036.590941
CAGTTAT270800.036.11260694
CGGACAC218700.033.9964812-13
Produced by FastQC (version 0.11.5)