FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12298203
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA645010.5244749985018137No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT579880.4715160418152148No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT309400.2515814708864376No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA229700.18677525488886465No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC229300.18645000411848786No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC226280.1839943608021432No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT210020.17077291698632718No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA205310.16694308916514064No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC203940.16582910527660016No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA175440.14265498788725475No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC145710.11848072437900074No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG143850.11696830829674872No Hit
AACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT138960.11299211762889261No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG133480.10853618207473076No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACGAGCTCAACAAGTGCAGTTT125990.10244586139942559No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG125070.101697784627559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT41000.037.7538221
GTATCGA43200.035.4612434
TATCGAA46750.033.472085
CGCGTAA35250.030.5243661
TGTCGAG11000.030.3309335
TCGTATG27750.028.79243742-43
GCGTAAC38350.028.5513422
CGGCATG43300.027.9996053
CGTAACT42650.025.8921973
GTATGCC32700.024.86513544-45
GTCGAGG13800.024.8578666
TATGCCG24650.024.59704646-47
CACGGAA42900.024.42894694
ACGACTT14700.024.398731
ACCCGTT98600.024.116242
GCCGTCT31700.023.42824648-49
ATCGAAG97900.022.6078016
CGTATCG92100.021.7865433
CGTATGC28400.021.67930844-45
CCGTATC92650.021.6580052
Produced by FastQC (version 0.11.5)