FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12298203
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC441630.35910124430374096No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA386180.31401335626026017No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT297500.24190526046772848No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT289990.23579867725390447No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC280650.22820407176560673No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA276470.22480520121516942No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT266810.2169503951105702No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT259970.21138860693712733No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC231050.18787297623888627No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG221590.18018079551947547No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG211880.1722853330685792No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT204100.16595920558475089No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC180240.14655799713177609No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT177760.14454144235544006No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC175720.14288266342651848No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG174480.14187438603835048No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA172510.14027252599424486No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT159070.12934410010958514No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC157200.1278235527580737No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA156610.12734380787176794No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA147390.1198467776145832No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG145820.11857016834085435No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT142390.11578114298487349No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG139490.11342307489964185No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG136200.11074788731329284No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT135670.11031693004254363No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA129400.10521862421688762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT18250.066.062791
GTCTAAC66400.063.834321
ACCGTTG21450.056.738983
GACCGTT22550.053.6738622
CGCAAAA47450.047.6414381
ACGTGCG98450.042.71688
CACCCGG64900.042.13934
AACACGT101550.041.7383845
CGTGCGC104200.040.269899
ACCCGGC69500.039.417845
TCACCCG71050.039.351563
ACACGTG108850.038.852716
GCGATTT65050.038.50234294
TCTAACA113950.037.816652
CACGTGC115000.036.5297747
TAACACG117650.035.866874
TCAGACG61500.035.761692
CCAGTAG54200.035.719635
CCGTTGA34800.034.9727334
CCGTCAT46700.034.489671
Produced by FastQC (version 0.11.5)