FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12270594
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC451050.3675861168579125No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA389190.31717290947773186No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT303150.24705405459588997No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT295190.24056700107590556No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC277500.2261504210798597No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA277460.2261178228209653No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT267030.21761782681425204No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT262340.2137956809588843No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC230590.1879208129614589No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG220980.1800890812620807No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG215350.17550087632269473No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT201220.16398554136825No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT182340.14859916317009592No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC181510.14792274929803725No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC178500.14546973031623409No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG174360.14209581052066428No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA173150.14110971318910887No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT158570.12922764782210217No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC158270.12898316088039422No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA158030.12878757132702784No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA152250.12407712291678791No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG143560.11699515117198075No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT142470.1161068486171085No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG139840.1139635130948021No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG138840.11314855662244223No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT133860.10909007339009015No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA131930.10751720739843565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT17150.069.941161
GTCTAAC62650.063.4679221
ACCGTTG20900.057.322933
GACCGTT22300.053.5775032
CGCAAAA46300.044.5721631
CACCCGG65000.043.7298774
ACGTGCG96200.041.0878038
GCGATTT63950.041.07590594
CCAGTAG57200.040.659935
TCACCCG71300.040.656513
ACCCGGC71150.039.9519465
AACACGT101200.039.2778135
CGTGCGC103550.038.2641879
CCGTTGA32200.036.9148334
ACACGTG108950.036.5700956
TCTAACA110950.036.1791572
TCAGACG65650.035.319422
TAGCATA68350.035.2771849
GTAGCAT68500.035.2666788
CACGTGC114550.034.704087
Produced by FastQC (version 0.11.5)