FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12262325
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA649290.5294999113137191No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT581420.4741515169431572No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT311780.25425847055921286No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA234620.19133402515428355No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC232680.18975194345281177No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC228850.1866285553514525No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC208940.17039183026057456No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA208640.1701471784510686No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT206740.1685977169908643No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA175430.14306422313876038No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC146520.11948794376270405No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG144740.1180363430263021No Hit
AACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT140640.11469276829638751No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG137050.11176510164263302No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACGAGCTCAACAAGTGCAGTTT127610.1040667247035126No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG126740.10335723445594534No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC124580.10159574142750254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT46600.032.272721
GTATCGA46200.031.5324754
TGTCGAG10550.029.8442485
TCGTATG24700.029.68716242-43
TATCGAA50500.029.405875
CGCGTAA34400.028.6430111
CGGCATG45650.028.4125463
GCGTAAC37150.026.9441432
ACCCGTT99350.026.4888922
GTATGCC30800.024.18998144-45
CGTAACT42200.024.053773
TATGCCG22850.023.86301246-47
GCCGTCT31150.022.7109148-49
CACGGAA43700.022.58612494
CACCCGT117750.022.5412651
GTCGAGG14200.022.1730166
ACGACTT13650.022.1043681
ATCGAAG99700.021.6820816
CCCGTTT128950.021.6110173
CGTATGC26400.020.92140844-45
Produced by FastQC (version 0.11.5)