FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13262918
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA42650.029.4173364
CCCGTAT44550.029.3452851
CGTATCG59100.024.1711433
ACGACTT20950.024.1049371
TATCGAA56550.023.7653665
CCGTATC60500.023.1473622
TCGACCG20550.022.64216630-31
CGGCATG46750.021.8116863
GTTCGAC21750.021.71765128-29
CGATCCG21550.021.15493682-83
ATCGAAG80450.020.793866
CGCGTAA29950.018.7523781
TCGAAGG93900.018.16577
AAGTTCG25550.017.7517726-27
ACGTACG5250.017.45898852-53
GCGTAAC33200.017.1272582
CGACTTT39300.016.979952
TGTCGAG11550.016.2738065
GCGAGCG40900.015.7449452-53
CGAGCGA34250.015.57682852-53
Produced by FastQC (version 0.11.5)