FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13208087
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT39800.030.4518571
GTATCGA40500.028.8908254
ACGACTT22100.023.686151
CGTATCG59800.023.574133
TATCGAA53250.022.8557625
CCGTATC62350.022.6853562
TCGACCG20950.021.53576730-31
CGGCATG44800.021.5026233
CGATCCG22200.020.64017182-83
GTTCGAC22300.020.33690628-29
CGTACGC13600.018.658235
ATCGAAG79000.017.9042026
ATCGCGT8950.017.85582794
TGTCGAG12350.017.5027565
AAGTTCG26850.017.3275426-27
CGCGTAT2203.1073298E-517.1486931
CGCGTAA29600.016.7286681
CCGTACG15550.016.318454
ACGTACG6700.015.78465652-53
GTTACGA7450.015.7688254
Produced by FastQC (version 0.11.5)