FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13208087
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC255600.19351780466012983No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT241640.18294852237118062No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT190560.1442752459156273No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT182100.13787007914166524No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC174730.13229016435158247No Hit
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG170270.12891344522488382No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG156880.11877571672566965No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT147710.11183300049431838No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG138530.10488271314384892No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT136930.10367133408494356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT23400.064.1746141
ACCGTTG29850.050.212183
GACCGTT31150.047.7536052
ACCCGGC65050.041.0270585
CCGTTGA40550.036.730864
GCGATTT68300.036.4635694
CACCCGG75900.035.1616554
TCACCCG76700.034.9174273
CGGCCCG82200.032.704968
CGTTGAC48000.032.0092775
CCAGTAG52750.030.3739475
CTACCGT40600.028.6128481
CATTCGG27900.028.460684
CGCAAAA59300.027.9514561
TAACCCC59400.027.2199759
ACATGCC100850.027.2053222
CCGTATG42200.026.8328844
CTCGATT62300.026.69896548-49
GCGGGTC54200.026.4867062
CACATGC104750.026.3275721
Produced by FastQC (version 0.11.5)