FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13164419
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT43450.032.0051041
GTATCGA41450.031.2905734
TATCGAA53650.025.2261925
CGTATCG60900.025.0781233
CCGTATC64050.024.6518272
ACGACTT21550.024.062041
TCGACCG22900.021.44802330-31
GTTCGAC23450.020.94370728-29
ATCGAAG78750.020.5275386
CGATCCG23750.020.0845882-83
CGTACGC16600.019.5330455
CGGCATG45200.019.0257423
CCGTACG17850.018.1651864
CGCGTAA28550.017.9973031
AAGTTCG27550.017.825426-27
TCGAAGG91600.017.5442527
TATCGCA27950.017.316488
GCGTAAC31350.016.7884042
TACGCCA20500.016.7329777
CGCGTAT2555.929218E-616.6375481
Produced by FastQC (version 0.11.5)