FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12694542
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC310250.244396371290906No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC225730.1778165766043391No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT205040.16151823358416553No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT183450.1445109244587162No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG179530.14142298320018162No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC169770.1337346396585241No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT162450.12796838200228097No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA151740.11953168534949903No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA150350.11843672658690642No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT146990.11578991979387677No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC143630.11314311300084715No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT139720.11006304914348229No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT127320.10029507169301578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT21300.066.336851
ACCGTTG27350.052.0574153
GACCGTT29550.047.4966962
CCGTTGA34250.041.8443224
CGCAAAA50050.038.018111
ACATGCC100850.033.204612
CACATGC105400.032.3527451
GCGATTT35150.031.68318794
CGTTGAC46600.030.553015
ACCCGGC42000.030.4310475
TAACCCC63350.029.3713289
CCTAGAG86250.028.9967021
CTACCGT47750.028.6047271
CCGTATG47500.027.5014
CGGCCCG46850.027.480178
TAGAGGC92650.026.6263223
GCCTATC127350.025.9388226
CTCGATT30050.025.40828748-49
CACCCGG51200.025.1465194
TGCCTAT132300.025.0750685
Produced by FastQC (version 0.11.5)