FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12658178
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG144860.11443985066413193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA49750.030.6028484
CCCGTAT51500.030.124591
CGTATCG63300.026.6502133
TATCGAA57800.026.4219865
CCGTATC65250.025.4216732
ATCGAAG81800.021.2548646
TCGACCG10650.020.74061830-31
CGGCATG30700.020.2043783
TCGAAGG90000.019.3705227
GTTCGAC11750.018.59862128-29
TATCGCA51150.017.7305248
TTATCGC51500.017.5187827
CGCGTAT1901.9744034E-417.3730851
CGCGTAA13100.017.2783571
ACGACTT15650.017.1748521
ACCCGTT30100.017.0164892
CTCGACT24750.016.7664071
CGTACGC13850.016.6247925
CGCTACT5700.016.5457951
CGAAGGC94200.015.9627978
Produced by FastQC (version 0.11.5)