FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12658178
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC308730.24389766046898692No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC222270.17559399148913848No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT208350.16459714818356955No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG181880.14368576583454587No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT179880.14210575961248134No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC168180.132862723213404No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT162090.12805160426721762No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA151310.11953537073028993No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT147070.1161857575395132No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA145760.11515085346406094No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC143410.11329434615313515No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT137460.10859382764249324No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT128920.10184720107427783No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG126980.10031459503887527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT22850.065.526041
ACCGTTG26400.057.1279563
GACCGTT30000.049.9089972
CCGTTGA34050.044.7069974
CGCAAAA50650.035.342771
GCGATTT34450.033.41923594
CGTTGAC47300.031.8863815
ACATGCC106100.031.7405912
CACATGC109800.031.2732451
ACCCGGC40650.031.2079015
CTACCGT41650.028.8044511
CGGCCCG44800.028.534758
TAACCCC64450.028.4412639
CCTAGAG86000.027.4058281
TAGAGGC87500.027.3311163
CATTCGG21850.026.8785694
CACCCGG48200.026.5136454
CCGTATG44500.026.395364
GCCTATC130850.025.7817296
TGCCTAT134400.025.1356985
Produced by FastQC (version 0.11.5)