FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13916311
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG170070.12220911130830577No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT60250.032.199421
GTATCGA59750.031.4588644
TATCGAA69200.027.9777035
CGTATCG73850.027.8068583
CCGTATC75900.027.3663642
CGCTAGT1859.607902E-925.5147631
CGCGTAT2151.7917046E-924.150021
ATCGAAG89250.023.5879786
TCGAAGG103550.020.512067
GTCCGAT6550.017.2955061
CGCTAAT3852.746674E-1017.1644781
TGTCGAG9900.016.1385565
CGAAGGC120500.015.9888718
CGCGTTT3302.4484143E-715.7341051
ACCCGTT51500.015.6948712
TTGCACG43200.015.5551188
CCGTTAA31900.015.02803630-31
CGCGTAG4401.8244464E-915.0189181
TTATCGC59700.014.9554777
TACCGCG32500.014.82165122-23
Produced by FastQC (version 0.11.5)