FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13819425
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG171230.12390529996725624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA53950.032.1387564
CCCGTAT56900.031.7300281
TATCGAA64050.028.0978975
CGTATCG69250.027.1415333
CCGTATC71050.026.5200582
ATCGAAG83850.022.863956
CGCGTAG5300.020.4567171
TCGAAGG99550.019.2099787
CGCGTAA6900.017.0794891
TCGGTCG12200.016.1757057
CTCGCGA2053.5346998E-416.0963761
CGGTAAT10700.015.85998251
CCGTTAA33150.015.45427930-31
TTGCACG47850.015.0239198
CGAAGGC117450.014.7620648
ACCCGTT47550.014.7241392
ACGGTTA35250.014.66247612-13
TATCGCA56800.014.5592188
TACCGCG35350.014.55632922-23
TTATCGC57300.014.5141767
Produced by FastQC (version 0.11.5)