FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14455154
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT619920.4288574165311556No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT364240.2519793286187058No Hit
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG295470.2044046019848699No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT266890.18463310733320448No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT241420.16701309442984835No Hit
CTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCTTTA238590.1650553152183643No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT217090.15018172756928083No Hit
CAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTT185520.12834176654223123No Hit
GTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCC176500.12210177767735993No Hit
GTTTCTTGGAGCTTCCACAAACTTAAAACCATGAAACATCTATTATTGCT171100.11836608589572965No Hit
CGTGCCTCCTGGTCTCAGTATGGCGCTGTCCTGGGTTCTTACAGTCCTGA169300.1171208553018529No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC167290.11573034780535717No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC161900.11200157397147066No Hit
GCAAGACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCT145350.10055237045554824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT12900.058.7482151
ACCGTTG21100.037.6376653
GACCGTT23050.033.0828252
CCGTTGA25050.031.1400154
ACCCGGC43500.030.1393175
CCGTATG31650.028.2096924
CGCAAAA53150.027.808971
GCGATTT46900.027.65503794
TAACCCC51650.027.1110539
CTACCGT38550.025.153671
TCACCCG53400.025.0796833
ACGTGCC53900.024.889412
GTTAGTT61650.024.5092751
CCGTCAT43150.023.99941
CGTATGG39100.023.4356125
CTCGATT36950.023.33649348-49
GTCTAAC16750.022.7629591
ACGCCTT282850.022.150342
CCAGTAG46400.021.875315
ATGACGG132300.021.7029894
Produced by FastQC (version 0.11.5)