FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004827678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14341298
Sequences flagged as poor quality0
Sequence length6
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCAA1397902197.47388974136094No Hit
NGTCAA755550.526835158156535No Hit
AGTTAA310930.21680743263266686No Hit
AGTCAG306880.2139834204686354No Hit
AGACAA282160.19674648696373231No Hit
AGTCAC270520.18863006681821967No Hit
AGTCCA245370.17109329992306135No Hit
ATTCAA212420.1481176947860647No Hit
AGCCAA206170.14375965132305318No Hit
AGGCAA162830.11353923473314619No Hit
CGTCAA147970.10317755059549004No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)