FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14341298
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA281550.19632114192174235No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG185410.12928397415631418No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT161830.11284194777906435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT28550.029.8905371
GTATCGA27750.027.4320834
CGTATCG47700.020.4904613
CCGTATC50900.020.0331272
TATCGAA49750.019.268325
ACCCGTT30000.018.3261572
TGTCGAG11400.017.7243525
CTTACTT80700.017.2933621
TATCGCA31700.016.750438
AATAAAG166200.016.68441894
ATCGAAG62850.016.5231846
CGCGTAA15000.016.3445641
ACGTACG5700.016.08056652-53
CGTACGC22800.016.0755755
TAATGCG10150.015.7406519
CTCGTTT64100.015.4462311
TTATCGC35000.015.4396197
ACGTTCG4903.45608E-1115.3437216
CGAGCGA19850.015.15517652-53
CGAAAGG47750.014.9581188
Produced by FastQC (version 0.11.5)