FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14341298
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT607800.42381101069094307No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT361330.2519506951183917No Hit
CCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCTCTGGGGACAG292300.20381697667812218No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT264120.1841674303120959No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT238460.16627504707035584No Hit
CTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCTTTA230690.16085712743713992No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT215820.15048847042994296No Hit
CAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTT185670.12946526876437545No Hit
GTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCC175120.12210889139881202No Hit
GTTTCTTGGAGCTTCCACAAACTTAAAACCATGAAACATCTATTATTGCT169810.11840629767263745No Hit
CGTGCCTCCTGGTCTCAGTATGGCGCTGTCCTGGGTTCTTACAGTCCTGA168330.11737431298059632No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC166080.11580541733391217No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC160080.11162169560942113No Hit
GCAAGACCAACCAAGATGAGTGCCCTGGGAGCTGTCATTGCCCTCCTGCT145250.10128093008038741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT13150.057.286291
ACCGTTG20100.038.573783
GACCGTT22400.033.420052
CCGTTGA23000.032.8928034
ACCCGGC45900.031.6336025
GCGATTT47450.029.61357594
CCGTATG33850.026.9305924
TCACCCG56200.026.6721943
GTTAGTT62000.026.198951
CCGTCAT42600.025.9725591
CGCAAAA51850.025.243671
CTACCGT39050.025.1989081
TAACCCC50000.024.8097929
CTCGATT36700.024.71207648-49
ACGTGCC54350.024.6889882
CATTCGG19250.024.166144
GCCTATC79300.023.4059756
ATGACGG130600.023.35378894
CGTATGG38850.023.1017675
GTCTAAC16350.022.4612181
Produced by FastQC (version 0.11.5)