FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14393734
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA282330.19614785155818498No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG188710.13110566028245346No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT166470.11565449243399942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT30200.031.8534811
GTATCGA28800.031.6551064
TATCGAA50200.021.9987835
CGTATCG52750.020.9353353
ACCCGTT29150.020.7962822
CCGTATC56250.019.3820632
ATCGAAG63950.019.3263456
TGTCGAG12050.019.1092455
CGCGTAA13900.018.9979571
AATAAAG173100.018.68059794
CGCTAGT2905.678703E-817.8866231
CGAAAGG48350.016.9115268
GTTCGAC11350.016.56249628-29
CACCCGT39650.016.5312351
CTTACTT80650.016.4883961
CCGTTTA43650.016.3641664
CGTACGA7250.016.208754-55
GCGTAAC17450.015.8888012
TCGACCG11900.015.79697630-31
TTCGAAA50650.015.6798456
Produced by FastQC (version 0.11.5)