FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12404840
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA201100.1621141425443617No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT197070.15886541059779893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA23000.037.104211
GCGTAAC26400.033.641332
CGTAACT37000.024.765563
CCCGTAT22000.022.5029281
GTATCGA19400.019.6199024
CACGGAA45250.019.1097494
GTAACTA53500.017.5666834
ACTAGTT57450.017.3399897
TAGTTAG56900.016.681779
CGCGTAT1700.001879861516.6408211
ACCCGTT59300.016.482532
CGTATCG38050.016.0547663
TAACTAG57000.015.9109465
GTTATCG28450.015.53556334-35
CTAGTTA60600.015.3530838
CGTTCGT31850.015.055284528-29
CCGTATC43800.014.9126892
CCGTTTA76100.014.8814734
CGTTTAC77400.014.7529495
TTATCGG32450.014.70729434-35
Produced by FastQC (version 0.11.5)