FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12346811
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA203820.16507906373556702No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT197910.16029240262931052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA21150.033.6671261
GCGTAAC24450.030.1742322
CGTAACT36500.020.8563143
GTATCGA18650.020.1570344
CCCGTAT21550.020.131681
CGTATCG36800.017.4939633
TGTCGAG15000.016.916795
CGGCATG32800.016.7620643
CACGGAA43900.016.05845394
TCGCGTA18500.015.4943562
GTAACTA51950.015.467714
CCGTATC43300.015.410472
ACTAGTT53150.015.3835977
ACCCGTT60050.015.0246262
TAGCGAC14650.014.91655626-27
TAACTAG54400.014.8574745
CGATTGG11250.014.6194484
TAGTTAG54200.014.5653859
TATCGAA30750.014.2119385
GTTATCG25750.014.14747434-35
Produced by FastQC (version 0.11.5)