FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12395513
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA199720.1611228192007866No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT199440.160896931010439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA23550.037.2452241
GCGTAAC26850.032.2035752
CGTAACT38600.023.3746193
GTATCGA20050.019.2189664
CCCGTAT23100.018.9853921
CACGGAA44150.018.62994294
CGTATCG39650.017.422273
ACTAGTT55400.017.3887927
GTAACTA53800.017.3820654
CGGCATG36500.016.4796373
GTTATCG26750.016.25165634-35
TAACTAG56300.016.1094045
TAGTTAG59600.015.6903329
CTAGTTA58950.015.3053318
ACCCGTT61500.015.1293642
CGTTCGT28950.015.09702128-29
CCGTATC48150.014.6394762
TCGTTAT29550.014.47275632-33
TTATCGG31250.014.13700734-35
TGTCGAG15050.014.0509795
Produced by FastQC (version 0.11.5)