FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12395513
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT238940.19276330072018802No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT161100.1299663838035586No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA128340.10353746553289081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT14250.042.275811
ACCGTTG18200.034.600273
GTCGTAA13200.034.1776055
TCGTAAG14650.031.1127286
GGTCGTA14550.031.006614
GCGATTT47250.029.83686694
GTCTAAC22200.028.6205271
TCACCCG55700.028.095393
ACCCGGC57000.028.0316315
GACCGTT22350.027.1651762
CACCCGG58100.027.015714
CGCGGTC18650.026.2452871
ACGTGCG23850.026.0072738
CCGTTGA25350.024.8412194
CAGACGT39000.024.3771861
CTTAGCG17550.024.0976478
CGACCTA10250.023.8410574
CGTTGAC27100.023.0635765
CTCGATT42450.022.97139548-49
TCAGACG27750.022.3877932
Produced by FastQC (version 0.11.5)