FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13937745
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA190210.13647114364626417No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT159740.11460964453001543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA21750.024.4992451
GCGTAAC25550.022.0700682
CGGCATG43450.018.7098243
GTATCGA19200.018.6005484
CCCGTAT23050.018.4121741
ACCCGTT51150.017.2713222
ATCGCGT14050.016.72560594
CGTAACT35900.016.0999323
TCGACCG10700.015.59299730-31
CGTATCG39700.015.1507043
GTTCGAC11200.014.89589228-29
CCGTATC45150.014.3626542
GTCCGAT15950.014.1910341
GAGCGAC38750.014.13147754-55
ATGCGCG59400.014.08408784-85
ATTCGCA38150.014.0447590-91
CGAGCGA29900.013.91250852-53
TGTCGAG16700.013.7877375
CGTTTAC75200.013.7473265
CCGTTTA73900.013.734814
Produced by FastQC (version 0.11.5)