FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13937745
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT157520.11301684741685258No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA146910.10540442517781749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT12250.035.3632771
GTCGTAA15550.031.4282785
TCGTAAG15650.031.2274576
GCGATTT52200.030.79006894
GTCTAAC25700.030.4141521
GGTCGTA16200.030.1672654
TCACCCG61850.028.5671773
ACCCGGC64900.026.934935
CTTAGCG20100.025.9499658
CACCCGG69900.025.4788424
ACGTGCG27300.025.1304388
GTTAGTT67800.024.0296341
ACCGTTG18700.023.1188073
CTCGATT47200.022.79883648-49
TTAGCGG24400.022.5323189
TAGCATA67800.022.317019
TAACACG31950.022.0616534
AGTACGC41650.022.05722848-49
CGCGGTC24100.021.882741
AACACGT34000.021.6989335
Produced by FastQC (version 0.11.5)