FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13946761
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT155050.11117276620715019No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA146750.1052215636304372No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT139890.10030285885016599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAA15950.031.5265665
GCGATTT50150.030.92195794
CGACCGT11400.030.5533371
TCGTAAG17700.028.4074946
TCACCCG60150.027.9694443
ACGTGCG26500.027.4859898
GTCTAAC27850.027.2102451
GGTCGTA19200.026.6786144
ACCCGGC62900.026.6729555
TAACACG29250.024.902554
CACCCGG68150.024.548344
CTTAGCG19600.024.2153538
GTTAGTT71500.023.1722621
CGCGGTC23600.023.135441
CTCGATT45650.022.75030148-49
AACACGT33200.022.5067425
ACCGTTG15550.022.3633983
CGACCTA12200.021.5707974
TTAGCGG23250.021.2222719
ATTCCGT49300.020.9235352-53
Produced by FastQC (version 0.11.5)