FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12259264
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT137250.11195615005925315No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA135540.11056128655031819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT22550.026.9689541
GTATCGA19500.025.7849754
CGCGTAA20650.022.1448421
CGGCATG42150.020.5135733
CGTATCG38750.020.2519043
CCGTATC43700.019.5709112
GCGTAAC25500.018.7966772
CGATTGG8800.016.5537574
TATCGAA34400.016.3923175
ATCGCGT11200.015.9451794
GAGCGAC37200.015.66495254-55
CGTAACT32800.015.3296073
CCCGTCG18250.015.240881
CGAGCGA30400.015.22766652-53
GCGACCA39800.015.05605156-57
ATTCGCA38300.014.96960990-91
CCCGTCT37600.014.5442421
ATGCGCG49350.014.18932884-85
GTATTAG61650.014.0248099
TAGCGAC11250.013.7852726-27
Produced by FastQC (version 0.11.5)