FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12259264
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT165080.134657349739756No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG150480.12274798878627623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT11500.045.432891
GTCGTAA15850.037.6525465
TCGTAAG16450.037.136196
GGTCGTA17350.034.397284
GCGATTT50400.033.00678694
ACCGTTG16250.031.5204943
TCACCCG54850.030.9279023
ACCCGGC56700.029.8359135
CGACCTA11300.029.1098164
CGCGGTC22550.028.596921
GACCGTT17650.028.5354232
CTTAGCG20450.028.4926158
CACCCGG63450.026.7359394
GTCTAAC21050.026.3860971
ACGTGCG21650.025.6110888
TTAGCGG23750.025.1274039
TAGCATA62250.025.0614519
CTCGATT45050.023.9913548-49
GTAGCAT68700.023.6659158
CCAGTAG64650.023.6229825
Produced by FastQC (version 0.11.5)