FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12165216
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT137850.11331488072221653No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA136200.11195855461999195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT22150.023.631181
CGCGTAA20900.022.1113851
GTATCGA20200.022.0995334
CGGCATG41900.021.9812773
CGCGTAT2601.6238118E-819.9505791
GCGTAAC24850.019.0987912
CGTATCG38600.018.8692783
TCGACCG12500.016.91977530-31
CCGTATC43750.016.75552
CGAGCGA30050.016.50224152-53
ACCCGTT35950.016.338872
GTTCGAC13300.015.90112928-29
TAGCGAC13600.015.55033726-27
GAGCGAC36850.015.43437654-55
TATCGAA32950.015.4020745
CGTAACT32200.015.3230183
ATCGCGT10150.015.28005294
ACGTTCG6800.015.2028386
GTATTAG62850.014.8783629
ATTCGCA38600.014.85469590-91
Produced by FastQC (version 0.11.5)