FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12165216
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT163680.13454754934067756No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG146810.12068014246520573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT13500.039.0674441
GTCGTAA14300.037.459115
TCGTAAG14550.036.492696
GGTCGTA17400.032.6758234
ACCGTTG17350.030.3325543
GCGATTT50900.029.2677394
ACCCGGC56100.028.1426535
TCACCCG56050.027.8325443
GACCGTT19500.027.2884042
ACGTGCG20300.026.6188138
CGACCTA13000.026.0243324
CACCCGG60800.025.5808434
CGCGGTC22550.025.2679231
TAGCATA63400.025.1994369
GTAGCAT63800.025.114278
CTCGATT42050.024.97638948-49
CCAGTAG60750.024.905675
GTCTAAC22500.023.6497541
CGTTGAC22900.023.5966855
CCGTTGA23350.022.7395574
Produced by FastQC (version 0.11.5)