FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12205893
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT138760.11368279240199794No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA135960.11138881849939206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT21500.022.3714871
GTATCGA18000.021.9299454
CGGCATG39400.020.8724083
CGCGTAA20800.020.6305681
CGTATCG36950.017.9322763
GCGTAAC23850.017.9301412
ACCCGTT31950.017.6498552
CCGTATC42650.015.9764292
TATCGAA31250.015.7895595
TCGACCG11950.015.33789330-31
CCGTTTA46000.015.0172444
TAGCGAC12500.014.85092326-27
ATTCGCA36200.014.73602390-91
CGACGGG14050.014.71773128-29
CGTTTAC48350.014.5789255
ACGTTCG5903.6379788E-1214.3367676
CGTACGC28000.014.2656535
GAGCGAC37800.014.238481554-55
CCGTACG28150.014.1896354
GTCCGAT15400.014.0854331
Produced by FastQC (version 0.11.5)