FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12205893
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT166920.13675361565106298No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG149430.122424471523714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT12650.045.7656361
GTCGTAA14700.034.527225
ACCGTTG17650.033.5491143
TCGTAAG15600.033.135736
GCGATTT49350.031.70987194
GGTCGTA16050.031.623194
TCACCCG56050.030.6874373
GACCGTT19600.029.7783932
ACCCGGC58750.029.2771225
CACCCGG64150.026.7394924
GTCTAAC21400.026.1732791
CGTTGAC23600.025.8872875
CCGTTGA24400.025.4238344
CGACCTA11650.025.010524
CTCGATT44850.024.41429748-49
CGCGGTC22900.024.2533361
CTTAGCG18900.024.1179398
ACGTGCG21800.023.2807798
CCGTATG24550.022.9713614
CCAGTAG59750.022.8094755
Produced by FastQC (version 0.11.5)