FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12181141
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT258410.2121394046748166No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG210280.1726275067335646No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA152380.12509501367729017No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC137600.11296150335998903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT15950.044.5563051
GTCGTAA12450.038.8762475
TCACCCG67300.037.3555533
ACCGTTG19650.035.6319963
ACCCGGC71950.035.3331955
CACCCGG73050.034.672494
GGTCGTA14350.034.38384
GCGATTT68250.032.6368594
GACCGTT21400.032.549242
TCGTAAG15050.032.472326
TAGCATA71100.032.3847929
GTCTAAC27950.031.6569391
CCAGTAG72950.030.9839135
GTAGCAT74700.030.9498968
CGACCTA10200.028.563274
GCCAGTA80100.028.2181824
ACGTGCG31250.027.517938
CGTTGAC25900.027.3964085
CAGTAGC85200.026.9151426
CGCGGTC19250.026.1604961
Produced by FastQC (version 0.11.5)