FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12112058
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA202250.16698235758118066No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT166470.13744154791861135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT20600.026.3209651
CGGCATG51500.023.995863
ACGACTT18200.023.833491
CGCGTAA29400.023.574431
GCGTAAC33300.021.5891232
TCGTATG18700.021.4972642-43
GTTCGAC15000.021.46232428-29
TCGACCG15400.020.9068930-31
TATGCCG16350.020.84516346-47
GTATCGA20150.020.7540454
TGTCGAG13150.018.5808435
ACCCGTT30350.018.5784872
CGATCCG16900.018.49386282-83
ATCGCGT17350.018.14832994
ACGTTCG7600.017.9296676
CGTAACT41250.017.656133
TATCGAA29500.016.4060125
GTATTAG74650.016.3026339
CGATTGG9300.016.1679464
AGTTTAC152200.016.02558994
Produced by FastQC (version 0.11.5)