FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12144059
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA204480.16837862859526623No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT168120.13843806259505162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA18650.023.685494
CGCGTAA28750.022.6394211
CGGCATG51600.022.4036483
TCGTATG18800.022.25246642-43
ACGACTT17750.021.7891161
CCCGTAT22550.021.7525861
TCGACCG15700.021.70193730-31
TATGCCG17700.021.11407746-47
GCGTAAC32950.020.537162
GTTCGAC16900.020.16097328-29
ATCGCGT15550.019.94854794
CGATCCG18550.018.24002882-83
CCCGTCG22000.018.0086331
CGTATCG43050.017.2471473
CGTAACT40050.017.1310373
ACCCGTT32500.016.9174862
TGTCGAG13150.016.4386225
CGTATGC22350.016.4053844-45
ATTCGCA48450.016.29677290-91
CGACGCA7500.016.2909134
Produced by FastQC (version 0.11.5)