FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13226848
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGC13200.026.6992685
CCCGTAT27350.026.2027631
GTATCGA24650.025.1633834
CCGTACG13850.025.106954
GTACGCC16150.022.4042426
CGTATCG39050.020.938483
CCGTATC44350.020.4494042
TACGCCA18350.019.717957
TCCGTAC19550.018.508063
CGCGTAA15050.018.1698911
TCGACCG11450.017.24635130-31
CGCTAGT3703.0395313E-916.565421
TATCGAA42900.016.430325
GTTCGAC12800.015.42559128-29
CCCGTCT39000.014.7487761
GCGTAAC18650.014.3619362
ACCCGTT23200.014.1783922
ATCGAAG65650.014.1007926
CGATCCG13650.013.94398582-83
CGATTGG10300.013.2303954
Produced by FastQC (version 0.11.5)