FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13128809
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGC13200.027.410145
CCCGTAT27400.027.0175691
CCGTACG13450.026.2019374
GTATCGA23300.026.0152634
GTACGCC17150.020.8230296
CGTATCG38200.020.7882423
CCGTATC43750.019.5473462
TACGCCA19350.018.4555537
TCCGTAC20900.017.7613183
CGCGTAA14650.016.7364311
TCGACCG10750.016.61404630-31
ACCCGTT25250.016.19022
TATCGAA42900.015.5534065
CGCGTGA3504.829344E-714.8190981
GTTCGAC12700.014.61738428-29
CGATTGG12150.014.3093484
ATCGAAG67100.014.0756346
CTCCGAT47350.013.6426191
CCCGTCG15950.013.5986041
CGATCCG12450.013.58937682-83
Produced by FastQC (version 0.11.5)