FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11491689
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG261210.22730340161485402No Hit
CTTACTTTCAACAAAATCAGCAGCTAATGAAGGCAAGTCAGCAGGCATCT152440.13265238904394297No Hit
CTTCAAATGGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAG152270.13250445604645236No Hit
GGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAGGCTTTGAA150310.13079887560479578No Hit
CAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACGAA143870.1251948255822099No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA137350.11952116003139313No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA127830.11123691217191833No Hit
CTCAGGTTCTTGTTTTGCACAGCAGTCAGCCATTTCACCATAGGTTTCAC126330.10993162101758931No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT124280.10814772310667299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT20050.029.3883341
GTATCGA18500.028.1938694
TGTCGAG10000.022.0851975
CGTATCG36350.020.553993
CTTACTT82050.020.5101551
CCGTATC40250.019.6141512
TATCGAA29250.019.5991575
GTCGAGG13550.016.9926136
ACGTACG6400.016.88948652-53
CGTACGA7100.015.88760254-55
ACCCGTT28750.015.6913192
GAAATAA159450.015.51298794
TAAGCCG105350.015.43552866-67
CGTACGC24000.015.0758875
TCGACCG4400.014.95129430-31
CGCGCAT3807.565177E-814.8859641
GACGAAA111200.014.64230446-47
ACTTTCA127800.014.5234444
TCGACGA113300.014.41216644-45
ATCGAAG49700.014.2765736
Produced by FastQC (version 0.11.5)