FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11489350
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT428470.3729279724266386No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT391600.3408373841862246No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT344170.29955567547337314No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT257630.2242337469047422No Hit
CCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTT183440.1596609033583275No Hit
GTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCC161790.14081736564731687No Hit
CTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCTTTA156430.13615217571054933No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT144060.1253856832631959No Hit
CAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTT143310.1247329048205512No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT136770.11904067680068933No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC136200.11854456518427936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT12350.047.9632761
ACCGTTG16900.034.2107933
CCGTTGA17400.033.4969944
GACCGTT18350.031.7677962
CCGTATG25350.025.4024724
GCGATTT20600.025.321894
CGACCTA8450.025.0316334
TAACCCC37050.023.3386659
CCCCACA189550.022.916771
GTCAACC82550.022.8366571
CATTCGG16600.022.6524564
CGTATGG28500.022.5928635
ACCCGGC27300.022.3808355
GCATTCG16950.022.1852843
GCCTATC64350.022.129076
CCCACAC207100.020.8383832
CCTAGAG60000.020.606761
CTTAGCG9950.020.309158
TAGAGGC59650.020.0155723
AACCCGT19350.019.9220642
Produced by FastQC (version 0.11.5)