FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12890254
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG234720.18209105887285076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT21850.024.8094831
GTATCGA17950.022.7750554
CGTATCG28500.020.2798563
TAAGCCG89650.017.19523866-67
CCGTATC36700.017.0298352
TCGACGA93850.016.39768444-45
GACGAAA95700.016.15437946-47
CCCGTCT35500.015.668411
CGAAACA99950.015.37374648-49
CCGAGCT105300.014.88623870-71
TATCGAA29700.014.7140135
TGTCGAG13350.014.4313585
CGTACGC25750.014.4163235
CCCAAAT224900.014.1476931
CCGTACG26300.014.114844
AGCCGAG110500.014.057610568-69
ATCGCGT8700.014.05315694
CGCTAAT4704.7148205E-914.0411111
CTCCGAT34150.013.9412571
CCCGAAT35100.013.6982261
Produced by FastQC (version 0.11.5)